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Sensitivity：2
Centrifuge the cell culture medium at room temperature (200 g) for 5 minutes
Th2-polarized cells
0 × 10 with serum-free medium6Cells/mL to prepare a cell suspension
Human IL-1β Surpass ELISA PairSet Kit Reagent Sensitivity：2Product Specification Antigen IL 1 Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Human Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 5. 0% Inter assay: 7. 0% Sample type Cell culture supernatant, SerumPlasma Assay type Sandwich(quantitative) Sensitivity 3. 884pg ml Range 15. 63pg mL 1000pg mL Recovery Cell culture supernatant: 85%
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