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It is necessary to conduct pre-experiments similar to cell number gradient detection
and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Plasminogen Activator and Urokinase (uPA)
and HRP conjugate are sequentially added to microwells pre-coated with a Transcription Factor A
Incubate the reaction at room temperature (22–25°C) for 40 minutes
UniOne® TR-FRET Human IL21/IL21R Binding Kit Size:2500T It is necessary to conductProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between IL21 and IL21R. Thismethod enables simple and rapid high throughput screening of inhibitors andantibody blockers. As illustrated, the interaction between IL21 and IL21R isdetected using an Eu labeled anti Tag1 antibody (TR FRET donor) and anAcceptor labeled anti Tag2 antibody
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